牛传染性鼻气管炎病毒gC蛋白单克隆抗体的制备与初步应用

doi:10.11843/j.issn.0366-6964.2013.10.018

Abstract

Abstract:

The objective of this study was to prepare monoclonal antibody against glycoprotein C (gC) protein of infectious bovine rhinotracheitis virus (IBRV) and to analyze its biological characteristic. BALB/c mice were immunized with purified recombinant gC protein expressed by E. coli and the spleen cells of immunized mouse were fused with SP2/0 myeloma cells. An indirect ELISA using the purified gC protein was developed to screen positive antibody-producing cells. A hybridoma stably secreting MAb designated as 3D6 was obtained against gC protein. The monoclonal antibody belongs to IgG1, and the light chain was κ. The titers of supernatant and ascites were 6.4×10³ and 1.28×10⁵ as detected by ELISA, respectively. The result of western blot assay and IFA suggested that the MAb could recognize IBRV. A sandwich ELISA was established by using 3D6 secreted monoclonal antibody. The results indicated that the ELISA possessed good specificity and higher sensitivity. The monoclonal antibody could be used to diagnose IBRV and further research gC protein function.

CLC Number:

S852.659.1

MA Hui, BIAN Chuan-zhou, WANG Yong-fen, WANG Xian-ping, ZHENG Bao-liang, ZHAO Xu-yong. Development and Preliminary Application of Monoclonal Antibody against IBRV gC and Identification of Its Biological Characteristic[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1637-1644.

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Development and Preliminary Application of Monoclonal Antibody against IBRV gC and Identification of Its Biological Characteristic

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